Antibody-based Proteomics for Human Tissue Profiling
نویسندگان
چکیده
منابع مشابه
Antibody-based proteomics for human tissue profiling.
Here, we describe the use of antibody-based proteomics involving the generation of protein-specific antibodies to functionally explore the human proteome. The antibodies can be used for analysis of corresponding proteins in a wide range of assay platforms, including i) immunohistochemistry for detailed tissue profiling, ii) specific affinity reagents for various functional protein assays, and i...
متن کاملTissue profiling of the mammalian central nervous system using human antibody-based proteomics.
A need exists for mapping the protein profiles in the human brain both during normal and disease conditions. Here we studied 800 antibodies generated toward human proteins as part of a Human Protein Atlas program and investigated their suitability for detailed analysis of various levels of a rat brain using immuno-based methods. In this way, the parallel, rather limited analysis of the human br...
متن کاملiTRAQ-based proteomics profiling of Schwann cells before and after peripheral nerve injury
Objective(s): Schwann cells (SCs) have a wide range of applications as seed cells in the treatment of nerve injury during transplantation. However, there has been no report yet on kinds of proteomics changes that occur in Schwann cells before and after peripheral nerve injury.Materials and Methods: Activated Schwann cells (ASCs) and normal Schwann cells (NSCs) were obtained from adult Wistar ra...
متن کاملSelection of Antigens for Antibody-based Proteomics
The human genome is predicted to contain ~20,500 protein-coding genes. The encoded proteins are the key players in the body, but the functions and localizations of most proteins are still unknown. Antibody-based proteomics has great potential for exploration of the protein complement of the human genome, but there are antibodies only to a very limited set of proteins. The Human Proteome Resourc...
متن کاملHuman Antibody Profiling with TruePlexTM Antibody Profiling Arrays Introduction
Many disease biomarkers are expressed at relatively low levels or are not secreted making them difficult to detect in serum or plasma. However, these biomarkers can stimulate a robust humoral immune response even if the antigens are expressed at very low levels. Because the immune system serves as a natural amplification system, antibodies to these biomarkers can be produced at detectable level...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2005
ISSN: 1535-9476
DOI: 10.1074/mcp.r500009-mcp200